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fiddlercrabfightingposewithfurmb3dmodel000.jpg Taken together, these outcomes suggest that LvRac1 is important in the innate immune response of shrimp to V. alginolyticus infection. Serine protease inhibitors, together with Kunitz-kind protease inhibitors play necessary roles not only in physiological process (i.e. blood clotting and fibrinolysis) but also in immune responses. On this examine, we characterized a Kunitz-kind protease inhibitor, designated MjKuPI, from kuruma shrimp Marsupenaeus japonicus. On this study, the cDNA of FREP was identified from the kuruma shrimp Marsupenaeus japonicus (MjFREP2). On this examine, a cDNA encoding a RAS GTPase (LvRac1) within the Pacific white shrimp (L. Injection of the bacterium V. alginolyticus into L. vannamei induced hepatopancreatic upregulation of LvRac1 expression. Suppressing LvRac1 also promoted the upregulation of Lvp53, LvCu/ZnSOD, and Lvgal following V. alginolyticus injection. The silencing of LvRac1 by double-stranded RNA injection thus elevated the V. alginolyticus challenge sensitivity of L. vannamei and weakened its bacterial clearance capacity in vivo. Moreover, knocking down LvRac1 in vivo significantly decreased the expression of the L. vannamei p53 and Cu/Zn superoxide dismutase genes (Lvp53 and LvCu/Zn SOD, respectively) while growing that of the galectin gene (Lvgal). What are good inquiries to ask a man while enjoying the question game?

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